7,710 research outputs found
Incorporation of a selective sigma-2 receptor ligand enhances uptake of liposomes by multiple cancer cells
Background: The sigma-2 receptor is an attractive target for tumor imaging and targeted therapy because it is overexpressed in multiple types of solid tumors, including prostate cancer, breast cancer, and lung cancer. SV119 is a synthetic small molecule that binds to sigma-2 receptors with high affinity and specificity. This study investigates the utility of SV119 in mediating the selective targeting of liposomal vectors in various types of cancer cells. Methods: SV119 was covalently linked with polyethylene glycol-dioleyl amido aspartic acid conjugate (PEG-DOA) to generate a novel functional lipid, SV119-PEG-DOA. This lipid was utilized for the preparation of targeted liposomes to enhance their uptake by cancer cells. Liposomes with various SV119 densities (0, 1, 3, and 5 mole%) were prepared and their cellular uptake was investigated in several tumor cell lines. In addition, doxorubicin (DOX) was loaded into the targeted and unmodified liposomes, and the cytotoxic effect on the DU-145 cells was evaluated by MTT assay. Results: Liposomes with or without SV119-PEG-DOA both have a mean diameter of approximately 90 nm and a neutral charge. The incorporation of SV119-PEG-DOA significantly increased the cellular uptake of liposomes by the DU-145, PC-3, A549, 201T, and MCF-7 tumor cells, which was shown by fluorescence microscopy and the quantitative measurement of fluorescence intensity. In contrast, the incorporation of SV119 did not increase the uptake of liposomes by the normal BEAS-2B cells. In a time course study, the uptake of SV119 liposomes by DU-145 cells was also significantly higher at each time point compared to the unmodified liposomes. Furthermore, the DOX-loaded SV119 liposomes showed significantly higher cytotoxicity to DU-145 cells compared to the DOX-loaded unmodified liposomes. Conclusion: SV119 liposomes were developed for targeted drug delivery to cancer cells. The targeting efficiency and specificity of SV119 liposomes to cancer cells was demonstrated in vitro. The results of this study suggest that SV119-modified liposomes might be a promising drug carrier for tumor-targeted delivery. © 2012 Zhang et al, publisher and licensee Dove Medical Press Ltd
Nanobiopesticides: Silica nanoparticles with spiky surfaces enable dual adhesion and enhanced performance
Biopesticides, such as spinosad, are a new-generation of ecofriendly pesticides in livestock industry. However, spinosad suffers from short duration of effectiveness and low potency in field conditions. Herein we report the development of a new nanospinosad design with dual adhesion and protection functions. Silica nanoparticles with spiky nanotopography loaded with spinosad possess rough surfaces. When applied topically, this nanospinosad formulation exhibited enhanced adhesion to both cattle hair and pest surface. The dual adhesion property led to significantly higher pest mortality toward tick (Rhipicephalus microplus, an ectoparasite) than a nanospinosad formulation using nanoparticles with smooth surface and a benchmark commercial product. The adhesion performance was further quantitatively measured using rainfastness test. Moreover, solar radiation test revealed that the nanospinosad exhibited >10 times higher photostability over the commercial product. This work paves the way toward the development of high performance nanobiopesticides for sustainable agricultural applications
SUCI02 inhibits the erbB-2 tyrosine kinase receptor signaling pathway and arrests the cell cycle in G 1 phase in breast cancer cells
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73321/1/j.1349-7006.2006.00143.x.pd
Airports at Risk: The Impact of Information Sources on Security Decisions
Security decisions in high risk organizations such as airports involve obtaining ongoing and frequent information about potential threats. Utilizing questionnaire survey data from a sample of airport
employees in European Airports across the continent, we analyzed
how both formal and informal sources of security information affect employee's decisions to comply with the security rules and
directives. This led us to trace information network flows to assess its impact on the degree employees making security decisions comply or deviate with the prescribed security rules. The results of the multivariate analysis showed that security information obtained through formal and informal networks differentially determine if employee will comply or not with the rules. Information sources emanating from the informal network tends to encourage employees to be more flexible in their security decisions
while formal sources lead to be more rigid with complying with rules and protocols. These results suggest that alongside the formal administrative structure of airports, there exists a diverse and pervasiveness set of informal communications networks that are a potent factor in determining airport security levels
Response to arXiv:0811.3876 "Comment on a recent conjectured solution of the three dimensional Ising model" by Wu et al
This is a Response to a recent Comment [F.Y. Wu et al., Phil. Mag. 88, 3093
(2008), arXiv:0811.3876] on the conjectured solution of the three-dimensional
(3D) Ising model [Z.D. Zhang, Phil. Mag. 87, 5309 (2007), arXiv:0705.1045].
Several points are made: 1) Conjecture 1, regarding the additional rotation, is
understood as performing a transformation for smoothing all the crossings of
the knots; 2) The weight factors in Conjecture 2 are interpreted as a novel
topologic phase; 3) The conjectured solution and its low- and high-temperature
expansions are supported by the mathematical theorems for the analytical
behavior of the Ising model. The physics behind the extra dimension is also
discussed briefly.Comment: 11 pages, 0 figure
Emergent excitations in a geometrically frustrated magnet
Frustrated systems are ubiquitous and interesting because their behavior is
difficult to predict. Magnetism offers extreme examples in the form of spin
lattices where all interactions between spins cannot be simultaneously
satisfied. Such geometrical frustration leads to macroscopic degeneracies, and
offers the possibility of qualitatively new states of matter whose nature has
yet to be fully understood. Here we have discovered how novel composite spin
degrees of freedom can emerge from frustrated interactions in the cubic spinel
ZnCr2O4. Upon cooling, groups of six spins self-organize into weakly
interacting antiferromagnetic loops whose directors, defined as the unique
direction along which the spins are aligned parallel or antiparallel, govern
all low temperature dynamics. The experimental evidence comes from a
measurement of the magnetic form factor by inelastic neutron scattering. While
the data bears no resemblance to the atomic form factor for chromium, they are
perfectly consistent with the form factor for hexagonal spin loop directors.
The hexagon directors are to a first approximation decoupled from each other
and hence their reorientations embody the long-sought local zero energy modes
for the pyrochlore lattice.Comment: 10 pages, 4 figures upon reques
High-density information storage in an absolutely defined aperiodic sequence of monodisperse copolyester
Synthesis of a polymer composed of a large discrete number of chemically distinct monomers in an absolutely defined aperiodic sequence remains a challenge in polymer chemistry. The synthesis has largely been limited to oligomers having a limited number of repeating units due to the difficulties associated with the step-by-step addition of individual monomers to achieve high molecular weights. Here we report the copolymers of ??-hydroxy acids, poly(phenyllactic-co-lactic acid) (PcL) built via the cross-convergent method from four dyads of monomers as constituent units. Our proposed method allows scalable synthesis of sequence-defined PcL in a minimal number of coupling steps from reagents in stoichiometric amounts. Digital information can be stored in an aperiodic sequence of PcL, which can be fully retrieved as binary code by mass spectrometry sequencing. The information storage density (bit/Da) of PcL is 50% higher than DNA, and the storage capacity of PcL can also be increased by adjusting the molecular weight (~38???kDa)
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